Effect of a monoclonal antibody against human relaxin-2 on cancer cell growth inhibition

被引：3

作者：

Park, Byoung Kwon ^{[1
,2
]}

Lee, Su In ^{[2
]}

Lee, Younghee ^{[3
]}

Cho, Sooyoung ^{[4
]}

Lee, Young Seek ^{[5
]}

Kwon, Hyung-Joo ^{[1
,2
]}

机构：

[1] Hallym Univ, Coll Med, Ctr Med Sci Res, Chunchon 24252, South Korea

[2] Hallym Univ, Coll Med, Dept Microbiol, Chunchon 24252, South Korea

[3] Chungbuk Natl Univ, Dept Biochem, Coll Nat Sci, Cheongju 28644, South Korea

[4] Seoul Natl Univ, Res Inst Vet Sci BK21, Lab Dev Biol & Genom, Coll Vet Med,Program Vet Sci, Seoul 08826, South Korea

[5] Hanyang Univ, Div Mol & Life Sci, Coll Sci & Technol, Ansan 15588, South Korea

来源：

APPLIED BIOLOGICAL CHEMISTRY | 2016年 / 59卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

Cancer; Monoclonal antibody; Relaxin-2; DNA-LIPOSOME COMPLEX; XENOGRAFT TUMOR-GROWTH; CPG-DNA; INCREASES SECRETION; CERVICAL GROWTH; IN-VIVO; PROGRESSION; EXPRESSION; PIG; OSTEOSARCOMA;

D O I：

10.1007/s13765-016-0221-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Relaxin-2 has gained much attention because of its importance in cancer cell proliferation, invasion, and angiogenesis. However, the potential of relaxin-2 as a target for immunotherapy has never been directly validated before. In the present study, we produced a human relaxin-2-specific antibody by immunizing mice using a specific liposome complex containing relaxin-2 epitope peptide and CpG-DNA. We isolated a hybridoma clone, 1B11F12, producing the anti-relaxin-2 monoclonal antibody. We confirmed with immunoprecipitation analysis that the antibody specifically recognizes native human relaxin-2. Treatment with the antibody suppressed the growth of some cancer cell lines. Therefore, we suggest that relaxin-2 could be a reasonable target of cancer therapeutics, and the monoclonal antibody could be used to treat cancer in humans.

引用

页码：739 / 746

页数：8

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