Kinetics of glycerol biotransformation to dihydroxyacetone by immobilized gluconobacter oxydans and effect of reaction conditions

被引：14
|
作者：
Raska, Jiri [1 ]
Skopal, Frantisek [1 ]
Komers, Karel [1 ]
Machek, Jaroslav [1 ]
机构：
[1] Univ Pardubice, Fac Chem Technol, Dept Phys Chem, CR-53210 Pardubice, Czech Republic
来源：
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS | 2007年 / 72卷 / 09期
关键词：
dihydroxyacetone; glycerol; biotransformations; kinetics; immobilized microorganisms; biotechnology;
D O I：
10.1135/cccc20071269
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Biotransformation of glycerol to 1,3-dihydroxyacetone was carried out in an isothermal isochoric batch reactor with Gluconobacter oxydans immobilized in poly( vinyl alcohol) gel capsules. The reaction course was described with a three-step kinetic model. Two reaction schemes were proposed and compared with 8 kinetic experiments at 25 degrees C. The experimental dependences of glycerol and dihydroxyacetone concentrations on reaction time were simulated very well by the autocatalytic model. The effects of reaction temperature and initial concentrations of yeast extract and glycerol were studied. Temperature 25 - 30 degrees C, initial yeast extract concentration 2 - 4 g l(-1) and initial glycerol concentration 20 - 50 g l(-1) were found as optimal. The determined rate constants can be used to advantage for industrial production of dihydroxyacetone from glycerol.
引用
收藏
页码：1269 / 1283
页数：15
相关论文
共 50 条
  • [1] Biotransformation of glycerol to dihydroxyacetone by recombinant Gluconobacter oxydans DSM 2343
    Gaetgens, Cornelia
    Degner, Ursula
    Bringer-Meyer, Stephanie
    Herrmann, Ute
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 76 (03) : 553 - 559
  • [2] Biotransformation of glycerol to dihydroxyacetone by recombinant Gluconobacter oxydans DSM 2343
    Cornelia Gätgens
    Ursula Degner
    Stephanie Bringer-Meyer
    Ute Herrmann
    Applied Microbiology and Biotechnology, 2007, 76 : 553 - 559
  • [3] Repeated use of immobilized Gluconobacter oxydans cells for conversion of glycerol to dihydroxyacetone
    Wei, Shenghua
    Song, Qingxun
    Wei, Dongzhi
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2007, 37 (01): : 67 - 76
  • [4] Monitoring of dihydroxyacetone production during oxidation of glycerol by immobilized Gluconobacter oxydans cells with an enzyme biosensor
    Tkác, J
    Navrátil, M
    Sturdík, E
    Gemeiner, P
    ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (4-5) : 383 - 388
  • [5] HYDROGEN-PEROXIDE AS AN OXYGEN SOURCE FOR IMMOBILIZED GLUCONOBACTER-OXYDANS CONVERTING GLYCEROL TO DIHYDROXYACETONE
    HOLST, O
    LUNDBACK, H
    MATTIASSON, B
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 22 (06) : 383 - 388
  • [6] GLYCEROL INHIBITION OF GROWTH AND DIHYDROXYACETONE PRODUCTION BY GLUCONOBACTER-OXYDANS
    CLARET, C
    BORIES, A
    SOUCAILLE, P
    CURRENT MICROBIOLOGY, 1992, 25 (03) : 149 - 155
  • [7] Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans
    D. Hekmat
    R. Bauer
    J. Fricke
    Bioprocess and Biosystems Engineering, 2003, 26 : 109 - 116
  • [8] Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans
    Hekmat, D
    Bauer, R
    Fricke, J
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2003, 26 (02) : 109 - 116
  • [9] Repeated biotransformation of glycerol to 1,3-dihydroxyacetone by immobilized cells of Gluconobacter oxydans with glycerol- and urea-feeding strategy in a bubble column bioreactor
    Hu, Zhong-Ce
    Tian, Sheng-Ying
    Ruan, Li-Juan
    Zheng, Yu-Guo
    BIORESOURCE TECHNOLOGY, 2017, 233 : 144 - 149
  • [10] Monitoring of the bioconversion of glycerol to dihydroxyacetone with immobilized Gluconobacter oxydans cell using thermometric flow injection analysis
    Navrátil, M
    Tkác, J
    Svitel, J
    Danielsson, B
    Sturdík, E
    PROCESS BIOCHEMISTRY, 2001, 36 (11) : 1045 - 1052
12345