Fluorescence Quenching and Enhancement of DNA-Templated Silver Nanocluster by DNA Hybridization

被引：3
|
作者：
Lee, Chunghyun [1 ]
Gang, Jongback [1 ]
机构：
[1] Gachon Univ, Dept Nano Chem, Sungnam 461701, Kyunggido, South Korea
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2017年 / 17卷 / 11期
关键词：
DNA-Templated Silver Nanocluster; Fluorescence Quenching; Electron Transfer; Mismatched Base; LABEL-FREE; ENDONUCLEASE ACTIVITY; BASE-PAIR; PROBE; DEACTIVATION; FLUOROPHORES; SELECTIVITY; INHIBITION; SEQUENCE;
D O I：
10.1166/jnn.2017.15077
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Oligonucleotide-templated Ag nanoclusters (DNA-AgNCs) has been widely used to detect biomolecules of interest due to the useful properties. To understand fluorescence quenching and enhancement of DNA-AgNCs by cDNA, this study used cDNA containing several mismatched bases (cDNA-1) and the full-length cDNA with a mismatched base (f-cDNA-1). We showed that DNA-AgNCs decreased fluorescence intensities to different degrees by hybridization of cDNA and cDNA-1. However, the fluorescence intensities of DNA-AgNCs in the presence of f-cDNA and f-cDNA-1 increased by 153% and 491%, respectively, compared to that of DNA-AgNCs, respectively.
引用
收藏
页码：7961 / 7965
页数：5
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