Qualitative and quantitative 1H NMR spectroscopy for determination of divalent metal cation concentration in model salt solutions, food supplements, and pharmaceutical products by using EDTA as chelating agent

被引：21

作者：

Hafer, Elina ^{[1
,2
]}

Holzgrabe, Ulrike ^{[2
]}

Kraus, Katharina ^{[1
]}

Adams, Kristie ^{[3
]}

Hook, James M. ^{[4
,5
]}

Diehl, Bernd ^{[1
]}

机构：

[1] Spectral Serv AG, Hoffmann Str 33, D-50996 Cologne, Germany

[2] Julius Maximilians Univ, Inst Pharm & Lebensmittelchem, Wurzburg, Germany

[3] Steelyard Analyt Inc, Gaithersburg, MD 20878 USA

[4] Univ New South Wales, Sch Chem, Sydney, NSW, Australia

[5] Univ New South Wales, Mark Wainwright Analyt Ctr, Sydney, NSW, Australia

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2020年 / 58卷 / 07期

关键词：

complexation; EDTA; food supplements; metal ions; NMR spectroscopy; pharmaceutical products; INORGANIC CATIONS; NMR; CALCIUM; MAGNESIUM; WATER;

D O I：

10.1002/mrc.5009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper introduces an H-1 NMR method to identify individual divalent metal cations Be2+, Mg2+, Ca2+, Sr2+, Zn2+, Cd2+, Hg2+, Sn2+, and Pb2+ in aqueous salt solutions through their unique signal shift and coupling after complexation with the salt of ethylenediaminetetraacetic acid (EDTA). Furthermore, quantitative determination applied for the divalent metal cations Ca2+, Mg2+, Hg2+, Sn2+, Pb2+, and Zn2+ (limit of quantification: 5-22 mu g/ml) can be achieved using an excess of EDTA with aqueous model salt solutions. An internal standard is not required because a known excess of EDTA is added and the remaining free EDTA can be used to recalculate the quantity of chelated metal cations. The utility of the method is demonstrated for the analysis of divalent cations in some food supplements and in pharmaceutical products.

引用

页码：653 / 665

页数：13