The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus

被引:18
|
作者
Wang, Manli [1 ,2 ,3 ]
Tan, Ying [1 ,2 ,3 ]
Yin, Feifei [1 ,2 ,3 ]
Deng, Fei [1 ,2 ]
Vlak, Just M. [4 ]
Hu, Zhihong [1 ,2 ]
Wang, Hualin [1 ,2 ]
机构
[1] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
[2] Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[4] Wageningen Univ, Virol Lab, NL-6709 PD Wageningen, Netherlands
来源
JOURNAL OF GENERAL VIROLOGY | 2008年 / 89卷
关键词
D O I
10.1099/vir.0.83466-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
F proteins of group II nucleopolyhedroviruses (NPVs) are envelope fusion proteins essential for virus entry and egress. An F-null Helicoverpa armigera single nucleocapsid NPV (HearNPV) bacmid, HaBac Delta F, was constructed. This bacmid could not produce infectious budded virus (BV) when transfected into HzAM1 cells, showing that F protein is essential for cell-to-cell transmission of BVs. When HaBac Delta F was pseudotyped with the homologous F protein (HaBac Delta F-HaF, positive control) or with the heterologous F protein from Spodoptera exigua multinucleocapsid NPV (SeMNPV) (HaBac Delta F-SeF), infectious BVs were produced with similar kinetics. In the late phase of infection, the BV titre of HaBac Delta F-SeF virus was about ten times lower than that of HaBac Delta F-HaF virus. Both pseudotyped viruses were able to fuse HzAM1 cells in a similar fashion. The F proteins of both HearNPV and SeMNPV were completely cleaved into F, and F2 in the BVs of vHaBac Delta F-HaF and vHaBac Delta F-SeF, respectively, but the cleavage of SeF in vHaBac Delta F-SeF- infected HzAM1 cells was incomplete, explaining the lower BV titre of vHaBac Delta F-SeF. Polyclonal antisera against HaF(1) and SeF1 specifically neutralized the infection of vHaBac Delta F-HaF and vHaBac Delta F-SeF, respectively. HaF(1) antiserum showed some cross-neutralization with vHaBac Delta F-SeF. These results demonstrate that group II NPV F proteins can be functionally replaced with a homologue of other group II NPVs, suggesting that the interaction of F with other viral or host proteins is not absolutely species-specific.
引用
收藏
页码:791 / 798
页数:8
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