Recent Developments in Open-Tubular Liquid Chromatography Columns

Liquid chromatography (LC) has traditionally been practiced using columns that are fully packed with discrete particles, or constructed around monolithic structures. The concept of open-tubular (OP) columns, or narrow internal diameter columns with a thin coating of stationary phase, has been known for many years. Limitations in sample capacity, column reproducibility, and instrumentation have hindered a broader adoption of the technique. However, recent developments in instrumentation, fabrication, nanoparticle technology, and other novel stationary phase supports have reignited activity in this area. Interest in open-tubular liquid chromatography (OTLC) stems from the potential for high efficiency, low backpressure, and the promise of more environmentally sustainable separations. This article explores recent developments in OTLC, with an emphasis on advances in column technology.