A method for regenerating gold surface for prolonged reuse of gold-coated surface plasmon resonance chip

被引：13

作者：

Vashist, Sandeep Kumar ^{[1
,2
]}

机构：

[1] Dublin City Univ, Natl Ctr Sensor Res, Ctr Bioanalyt Sci CBAS, Dublin 9, Ireland

[2] Bristol Myers Squibb BMS, Swords Labs, Swords, Co Dublin, Ireland

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 423卷 / 01期

关键词：

Regeneration; Gold surface; Surface plasmon resonance; Piranha solution; APTES; EDC; Sulfo-NHS; Human fetuin A immunoassay; GLUCOSE-OXIDASE; BIOSENSOR; TECHNOLOGY; SENSORS; ELISA; FUNCTIONALIZATION; IMMUNOASSAY; ADSORPTION; STRATEGIES; HAPTEN;

D O I：

10.1016/j.ab.2011.12.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a method to completely regenerate the gold (Au) surface of 3-aminopropyltriethoxysilane (APTES)-functionalized Au-coated surface plasmon resonance (SPR) chip that had been used for human fetuin A (HFA) immunoassay. It involved treatment of the used SPR chip with freshly prepared piranha solution (concentrated H2SO4/30% H2O2 = 3:1, v/v) for 15 min followed by extensive rinsing with ethanol and deionized water. The developed method enabled prolonged reuse of the regenerated SPR chip that increased its cost-effectiveness without affecting the reproducibility of HFA immunoassays. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：23 / 25

页数：3

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