Trans-resveratrol, a red wine ingredient, inhibits voltage-activated potassium currents in rat hippocampal neurons

被引:50
|
作者
Gao, ZB [1 ]
Hu, GY [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Media, State Key Lab Drug Res, Shanghai 201203, Peoples R China
基金
中国国家自然科学基金;
关键词
antioxidant; hippocampus; neuroprotection; trans-resveratrol; voltage-activated K+ current;
D O I
10.1016/j.brainres.2005.07.013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The red wine ingredient traps-resveratrol was found to exert potent neuroprotective effects in different in vivo and in vitro models. Thus far, the mechanisms underlying the neuroprotection were attributed mainly to its antioxidant properties. The aim of this study was to investigate the actions of traps-resveratrol on voltage-gated K+ channels, which have been implicated in neuronal apoptosis. Superfusion of traps-resveratrol reversibly inhibited both the delayed rectifier (I-K) and fast transient K+ current (I-A) in rat dissociated hippocampal neurons with IC50 values of 13.6 +/- 1.0 mu M and 45.7 +/- 7.5 mu M, respectively. The inhibition on IK had a slow onset, was neither voltage dependent nor use dependent. Traps-resveratrol (30 mu M) shifted the steady-state inactivation curve of IK to the hyperpolarizing direction by 20 mV and slowed down its recovery from inactivation. The inhibition on I-A was similar to that on IK, but voltage dependent. Superfusion of transresveratrol (30 mu M) shifted the steady-state activation curve of IA to the depolarizing direction by 17 mV. Intracellular application of transresveratrol (30 mu M) was ineffective. Based on the comparable effective concentrations, the inhibition of voltage-activated K+ currents by trans -resveratrol may contribute to its neuroprotective effects. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:68 / 75
页数:8
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