Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury

被引：3
|
作者：
Yu, Jiangtao [1 ]
Chen, Haihua [1 ,2 ]
Ma, Haoli [3 ]
Zhang, Zhongxiang [1 ]
Zhu, Xiaolu [1 ]
Wang, Pengcheng [1 ]
Liu, Ruining [1 ]
Jin, Xiaoqing [1 ,2 ]
Zhao, Yan [1 ,2 ]
机构：
[1] Wuhan Univ, Emergency Ctr, Zhongnan Hosp, Wuhan, Peoples R China
[2] Wuhan Univ, Hubei Clin Res Ctr Emergency & Resuscitat, Zhongnan Hosp, Wuhan, Peoples R China
[3] Wuhan Univ, Dept Biol Repositories, Zhongnan Hosp, Wuhan, Peoples R China
来源：
FRONTIERS IN NEUROSCIENCE | 2022年 / 16卷
关键词：
traumatic spinal cord injury; mRNA; methylation; epigenetic; MeRIP-Seq; m6A (N6-methyladenosine); RNA METHYLATION; EPIDEMIOLOGY; DEMOGRAPHICS; NEURONS; DEATH;
D O I：
10.3389/fnins.2022.848119
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Recent studies showed that RNA N6-methyladenosine (m6A) plays an important role in neurological diseases. We used methylated RNA immunoprecipitation sequencing (MeRIP-Seq) technology to generate the m6A modification map after traumatic spinal cord injury (TSCI). A total of 2,609 differential m6A peaks were identified after TSCI. Our RNA sequencing results after TSCI showed 4,206 genes with significantly altered expression. Cross-link analysis of m6A sequencing results and RNA sequencing results showed that 141 hyper-methylated genes were upregulated, 53 hyper-methylated genes were downregulated, 57 hypo-methylated genes were upregulated, and 197 hypo-methylated genes were downregulated. Among these, the important inflammatory response factor Tlr4 and the important member of the neurotrophin family Ngf were both upregulated and hyper-methylated after TSCI. This study provides that in the future, the epigenetic modifications of the genes could be used as an indicator of TSCI.
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页数：14
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