Enhancement of Mouse Serum Cytokine Production by Non-degassed Roasted Coffee Bean Extracts

In order to clarify the functional difference of coffee bean extracts which non-degassed or degassed, we examined the effect on mouse immune function of the roasted coffee bean extracts degassed for 0-96 h. The commercial roasted coffee beans are commonly degassed. We previously found that large quantity of volatile compounds in coffee extracts were decreased by the degassing. In this study, we found that the extracts of non degassed roasted coffee beans were enhanced serum IFN-gamma, IL-2, and IL-12 production of mice, in comparison with other degassed coffee. Moreover, we found that 2 kinds of sulfur compounds and 3 kinds of ketone compounds which were decreased by the degassing, enhanced serum IFN-gamma production of mice. In addition, IL-2 production was enhanced by 2 kinds of sulfur compounds and 2 kinds of pyrazine compounds, and IL-12 production was enhanced by 4 kinds of pyrazine compounds. These results concluded that the roasted coffee beans which was shortened degassing time within 30 hours, hold enough volatile compounds for enhancing serum IFN-gamma, IL-2, and IL-12 production of mice.