Neuroglycan C, A Brain-Specific Chondroitin Sulfate Proteoglycan, Interacts with Pleiotrophin, A Heparin-Binding Growth Factor

被引：16
|
作者：
Nakanishi, Keiko [1 ]
Tokita, Yoshihito [1 ]
Aono, Sachiko [1 ]
Ida, Michiru [1 ]
Matsui, Fumiko [1 ]
Higashi, Yujiro [1 ]
Oohira, Atsuhiko [2 ]
机构：
[1] Aichi Human Serv Ctr, Inst Dev Res, Dept Perinatol, Aichi 4800392, Japan
[2] Aichi Med Univ, Aichi, Japan
来源：
NEUROCHEMICAL RESEARCH | 2010年 / 35卷 / 08期
基金：
日本学术振兴会;
关键词：
Neuroglycan C; Pleiotrophin; Neurite outgrowth; Chondroitin sulfate proteoglycan; Heparin-binding growth factor; MOLECULE HB-GAM; PHOSPHATASE ZETA/RPTP-BETA; PART-TIME PROTEOGLYCAN; LONG-TERM POTENTIATION; NEURITE OUTGROWTH; TENASCIN-R; EGF FAMILY; IN-VIVO; PROTEIN; IDENTIFICATION;
D O I：
10.1007/s11064-010-0164-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-band protein recognized by an anti-pleiotrophin antibody. The binding of pleiotrophin (PTN) to NGC was confirmed by a quartz crystal microbalance method and had a Kd of 8.7 nM. PTN bound to the acidic amino acid cluster of the NGC extracellular domain. In addition, PTN bound to both chondroitin sulfate-bearing NGC and chondroitinase-treated NGC prepared from the neonatal rat brain. These results suggest that NGC interacts with PTN.
引用
收藏
页码：1131 / 1137
页数：7
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