Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres

被引：9

作者：

Mykkanen, A. K. ^{[1
]}

Hyyppa, S. ^{[2
]}

Poso, A. R. ^{[1
]}

Roneus, N. ^{[3
]}

Essen-Gustavsson, B. ^{[4
]}

机构：

[1] Univ Helsinki, Dept Vet Basic Sci, FIN-00014 Helsinki, Finland

[2] Agrifood Res Finland, FIN-32100 Ypaja, Finland

[3] Hastsjukhuset Solvalla, S-16102 Bromma, Sweden

[4] Swedish Univ Agr Sci, Dept Clin Sci, SE-75007 Uppsala, Sweden

来源：

RESEARCH IN VETERINARY SCIENCE | 2010年 / 89卷 / 03期

关键词：

Horse; Muscle; Lactate; MCT1; CD147; Immunohistochemistry; HEAVY-CHAIN ISOFORMS; MONOCARBOXYLATE TRANSPORTERS; LACTATE; TISSUES; HORSES; LOCALIZATION; MITOCHONDRIA; EXPRESSION; MEMBRANE;

D O I：

10.1016/j.rvsc.2010.03.026

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Monocarboxylate transporter 1 (MCT1) and its ancillary protein CD147 facilitate efflux of lactate from the muscle. Expression of MCT-1 and CD147 were studied with immunohistochemistry in type I, IIA, IIAB and IIB fibres of equine gluteal muscle. Staining intensity of MCT1 in the cytoplasm as well as in the membranes of fibre types decreased in the order I = IIA > IIAB > IIB and correlated with the oxidative capacity. Capillaries were pronounced in the MCT1 staining. CD147 antibody stained plasma membranes of all fibre types evenly, whereas the staining in the cytoplasm followed that of MCT1. In the middle gluteal muscle the expression of MCT1 follows the oxidative capacity of muscle fibres, but the expression of CD147 in sarcolemma does not vary among fibre types. The use of horse specific MCT1 and CD147 antibodies can in future studies help to evaluate lactate efflux from different muscle fibre types. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：432 / 437

页数：6

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