Top-down mass spectrometry on low-resolution instruments: Characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways
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作者:
Meluzzi, Dario
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Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USAUniv Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Meluzzi, Dario
[3
]
Zheng, Wei Hao
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机构:
Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USAUniv Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Zheng, Wei Hao
[3
]
Hensler, Mary
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机构:
Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USAUniv Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Hensler, Mary
[2
]
Nizet, Victor
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USAUniv Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Nizet, Victor
[1
,4
]
Dorrestein, Pieter C.
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Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USAUniv Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
Dorrestein, Pieter C.
[1
,2
,3
]
机构:
[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA
Mass spectrometry (MS) is an important tool for studying non-ribosomal peptide, polyketide, and fatty acid biosynthesis. Here we describe a new approach using multi-stage tandem MS on a common ion trap instrument to obtain high-resolution measurements of the masses of substrates and intermediates bound to phosphopantetheinylated ( holo) carrier proteins. In particular, we report the chemical formulas of 12 diagnostic MS(3) fragments of the phosphopantetheine moiety ejected from holo carrier proteins during MS(2). We demonstrate our method by observing the formation of holo-AcpC, a putative acyl carrier protein from Streptococcus agalactiae. Published by Elsevier Ltd.
机构:
Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R ChinaSun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
Zhang, Ying
Cai, Qinghua
论文数: 0引用数: 0
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机构:
Henan Univ, Sch Life Sci, Henan Engn Lab Mammary Bioreactor, Kaifeng 475004, Henan, Peoples R ChinaSun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
Cai, Qinghua
Luo, Yuxiang
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机构:
Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R ChinaSun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
Luo, Yuxiang
Zhang, Yu
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机构:
Shennong Lab, Zhengzhou 450002, Peoples R ChinaSun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
Zhang, Yu
Li, Huilin
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机构:
Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Peoples R ChinaSun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
机构:
Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
Zhang, Jiang
Loo, Rachel R. Ogorzalek
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机构:
UCLA, David Geffen Sch Med, Mol Biol Inst, Dept Biol Chem, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, UCLA DOE, Inst Genom & Prote, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
Loo, Rachel R. Ogorzalek
Loo, Joseph A.
论文数: 0引用数: 0
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机构:
Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
UCLA, David Geffen Sch Med, Mol Biol Inst, Dept Biol Chem, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, UCLA DOE, Inst Genom & Prote, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
机构:
Merck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
Boston Univ, Dept Biochem, Ctr Biomed Mass Spectrometry, Sch Med, Boston, MA 02118 USAMerck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
Yu, Xiang
Warme, Christopher
论文数: 0引用数: 0
h-index: 0
机构:
Merck Res Labs, Pharmaceut Sci, Analyt Sci, Summit, NJ 07901 USAMerck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
Warme, Christopher
Lee, Dinah
论文数: 0引用数: 0
h-index: 0
机构:
Merck Res Labs, Pharmaceut Sci, Analyt Sci, Summit, NJ 07901 USAMerck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
Lee, Dinah
Zhang, Jing
论文数: 0引用数: 0
h-index: 0
机构:
Merck Res Labs, Pharmaceut Sci, Analyt Sci, Summit, NJ 07901 USAMerck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
Zhang, Jing
Zhong, Wendy
论文数: 0引用数: 0
h-index: 0
机构:
Merck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USAMerck Res Labs, Struct Elucidat Grp, Global Proc & Analyt Chem, Summit, NJ 07901 USA
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Dept Physiol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Xu, Fangmin
Xu, Qingge
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Dept Physiol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Xu, Qingge
Dong, Xintong
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h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Dong, Xintong
Guy, Moltu
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h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Guy, Moltu
Guner, Huseyin
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机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Dept Physiol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Guner, Huseyin
Hacker, Timothy A.
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机构:
Univ Wisconsin, Sch Med & Publ Hlth, Dept Med, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Hacker, Timothy A.
Ge, Ying
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA
Univ Wisconsin, Sch Med & Publ Hlth, Dept Physiol, Madison, WI 53706 USAUniv Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA