Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

被引:4
|
作者
Li, Guoxiu [1 ]
Wubshet, Ashenafi Kiros [1 ,2 ]
Ding, Yaozhong [1 ]
Li, Qian [1 ]
Dai, Junfei [1 ]
Wang, Yang [1 ]
Hou, Qian [1 ]
Chen, Jiao [1 ]
Ma, Bing [1 ]
Szczotka-Bochniarz, Anna [3 ]
Szathmary, Susan [4 ]
Zhang, Yongguang [1 ]
Zhang, Jie [1 ]
机构
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl OIE Foot & Mouth Dis Reference Lab, Lanzhou 730046, Peoples R China
[2] Mekelle Univ, Dept Basic & Diagnost Sci, Coll Vet Sci, Tigray 280, Ethiopia
[3] Natl Inst Vet Res, Dept Swine Dis, 57 Partyzantow, PL-24100 Pulawy, Poland
[4] RT Europe Res Ctr, H-9200 Mosonmagyarovar, Hungary
来源
VIRUSES-BASEL | 2021年 / 13卷 / 06期
基金
国家重点研发计划;
关键词
FMDV; SAT2; structural proteins; antigenicity; immunogenicity; vaccine; MOUTH-DISEASE VIRUS; OLIGOSACCHARIDE RECEPTOR; ESCHERICHIA-COLI; PROTECTION; SEROTYPE; EVOLUTION; CATTLE; SITES; SWINE; SUMO;
D O I
10.3390/v13061005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.
引用
收藏
页数:14
相关论文
共 24 条
  • [1] Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
    Fei, Dongliang
    Zhang, Haochun
    Diao, Qingyun
    Jiang, Lili
    Wang, Qiang
    Zhong, Yi
    Fan, Zhaobin
    Ma, Mingxiao
    PLOS ONE, 2015, 10 (06):
  • [2] Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 (vol 10, e0128486, 2015)
    Fei, Dongliang
    Zhang, Haochun
    Diao, Qingyun
    Jiang, Lili
    Wang, Qiang
    Zhong, Yi
    Fan, Zhaobin
    Ma, Mingxiao
    PLOS ONE, 2015, 10 (07):
  • [3] FORMATION OF POLIOVIRUS-LIKE PARTICLES BY RECOMBINANT BACULOVIRUSES EXPRESSING THE INDIVIDUAL VP0, VP3 AND VP1 PROTEINS BY COMPARISON TO PARTICLES DERIVED FROM THE EXPRESSED POLIOVIRUS POLYPROTEIN
    BRAUTIGAM, S
    SNEZHKOV, E
    BISHOP, DHL
    VIROLOGY, 1993, 192 (02) : 512 - 524
  • [4] FOOT-AND-MOUTH-DISEASE VIRUS CAPSID PROTEINS VP0, VP1 AND VP3 SYNTHESIZED BY INVITRO TRANSLATION ARE THE MAJOR COMPONENTS OF 14S PARTICLES
    GRIGERA, P
    VASQUEZ, C
    PALMENBERG, A
    ACTA VIROLOGICA, 1985, 29 (06) : 449 - &
  • [5] Immunogenicity analysis of the E. coli expressed structural protein VP1 of persistent infection foot-and-mouth disease virus
    Tang, Huan
    Wang, Hailong
    Yang, Li
    Chen, Hong
    Kong, Lingbao
    Xin, Xiu
    VIROLOGY, 2023, 579 : 111 - 118
  • [6] Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus
    Hiremath, C.
    Jhala, M. K.
    Bhanderi, B. B.
    Joshi, C. G.
    IRANIAN JOURNAL OF VETERINARY RESEARCH, 2013, 14 (04) : 354 - 357
  • [7] CHARACTERIZATION OF MESSENGER-RNAS FOR POLYOMA-VIRUS CAPSID PROTEINS VP1, VP2, AND VP3
    HUNTER, T
    GIBSON, W
    JOURNAL OF VIROLOGY, 1978, 28 (01) : 240 - 253
  • [8] Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV)
    Anthony, S. J.
    Maan, N.
    Maan, S.
    Sutton, G.
    Attoui, H.
    Mertens, P. P. C.
    VIRUS RESEARCH, 2009, 145 (02) : 187 - 199
  • [9] A VIABLE SIMIAN VIRUS-40 VARIANT WITH A DELETION IN THE OVERLAPPING GENES FOR VIRION PROTEINS VP1, VP2 AND VP3
    NORKIN, LC
    PIATAK, M
    JOURNAL OF GENERAL VIROLOGY, 1982, 63 (DEC): : 513 - 516
  • [10] EXPRESSION OF THE POLYOMAVIRUS MINOR CAPSID PROTEINS VP2 AND VP3 IN ESCHERICHIA-COLI - IN-VITRO INTERACTIONS WITH RECOMBINANT VP1 CAPSOMERES
    DELOS, SE
    CRIPE, TP
    LEAVITT, AD
    GREISMAN, H
    GARCEA, RL
    JOURNAL OF VIROLOGY, 1995, 69 (12) : 7734 - 7742