Oryzihumus soli sp nov., isolated from soil and emended description of the genus Oryzihumus

A novel strain designated Aerobe-19(T) was isolated from a soil sample collected from a lawn located in Seoul National University in Korea. The cells were Gram-stain-positive, aerobic, and coccus-shaped. Colonies were circular with entire edges, convex, opaque and pale yellow. The strain grew at 15-30 degrees C (optimum, 30 degrees C), pH 5.0-7.0 (optimum, 6.0) and in the presence of 0-0.5% (w/v) NaCl. Phylogenetically, the strain was found to be closely related to members of the genus Oryzihumus and showed 16S rRNA gene sequence similarities of 96.4 and 96.3% with Oryzihumus leptocrescens NRRL B-24347(T) and Oryzihumus terrae KACC 16543(T), respectively. The major cellular fatty acids were anteiso-C-17:0, iso-C-15:0, anteiso-C-15:0 and iso-C-16:0. The predominant menaquinone was MK-8 (H-4). The polar lipids profile revealed the presence of diphosphatidylglycerol, glycolipids, unknown amino-glycophospholipid, unknown phospholipid and unknown lipids. The peptidoglycan type was A1 gamma. The DNA G+C content of this stain was 73.9 mol%. On the basis of data presented, strain Aerobe-19(T) is considered to represent a novel species of the genus Oryzihumus, for which the name Oryzihumus soli sp. nov. is proposed. The type strain is Aerobe-19(T) (=KACC 18485(T) = KCTC 39705(T) =NBRC 111450(T)).