Intestinal lactase-phlorizin hydrolase (LPH):: the two catalytic sites;: the role of the pancreas in pro-LPH maturation

被引：42
|
作者：
Zecca, L
Mesonero, JE
Stutz, A
Poirée, JC
Giudicelli, J
Cursio, R
Gloor, SM
Semenza, G [1 ]
机构：
[1] Swiss Fed Inst Technol, ETH Zentrum, Dept Biochem, CH-8092 Zurich, Switzerland
[2] Univ Milan, Dipartimento Chim & Biochim Med, Milan, Italy
[3] Univ Nice, Fac Med, Biochim Lab, F-06107 Nice, France
[4] Univ Nice, Fac Med, Chirurg Expt Lab, F-06107 Nice, France
来源：
FEBS LETTERS | 1998年 / 435卷 / 2-3期
关键词：
lactase; active site; proteolytic processing; pancreas;
D O I：
10.1016/S0014-5793(98)01076-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Brush border lactase-phlorizin hydrolase carries two catalytic sites. In the human enzyme lactase comprises Glu-1749, phlorizin hydrolase Glu-1273. The proteolytic processing of pro-lactase-phlorizin hydrolase by (rat) enterocytes stops two amino acid residues short of the N-terminus of 'mature' final, brush border lactase-phlorizin hydrolase. Only these two amino acid residues are removed by luminal pancreatic protease(s), probably trypsin. (C) 1998 Federation of European Biochemical Societies.
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收藏
页码：225 / 228
页数：4
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