The study of multivalent carbohydrate-protein interactions by bio-layer interferometry

被引：25

作者：

Laigre, Eugenie ^{[1
]}

Goyard, David ^{[1
]}

Tiertant, Claire ^{[1
]}

Dejeu, Jerome ^{[1
]}

Renaudet, Olivier ^{[1
]}

机构：

[1] Univ Grenoble Alpes, CNRS, DCM UMR 5250, F-38000 Grenoble, France

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2018年 / 16卷 / 46期

基金：

欧洲研究理事会;

关键词：

LECTIN; AFFINITY; DESIGN; GLYCODENDRIMERS; GLYCOMIMETICS; GLYCOCLUSTERS;

D O I：

10.1039/c8ob01664j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The study of complex multivalent carbohydrate-protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra-and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interactions going from the micro to the nanomolar range.

引用

页码：8899 / 8903

页数：5

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