Bioinformatics and Microarray Analysis of miRNAs in Aged Female Mice Model Implied New Molecular Mechanisms for Impaired Fracture Healing

被引：21

作者：

He, Bing ^{[1
,2
]}

Zhang, Zong-Kang ^{[3
]}

Liu, Jin ^{[1
,2
]}

He, Yi-Xin ^{[1
]}

Tang, Tao ^{[4
]}

Li, Jie ^{[3
]}

Guo, Bao-Sheng ^{[1
,2
]}

Lu, Ai-Ping ^{[1
,2
]}

Zhang, Bao-Ting ^{[3
]}

Zhang, Ge ^{[1
,2
]}

机构：

[1] Hong Kong Baptist Univ, Inst Adv Translat Med Bone & Joint Dis, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China

[2] HKBU Shenzhen Res Inst & Continuing Educ, Inst Integrated Bioinformed & Translat Sci, Shenzhen 518000, Peoples R China

[3] Chinese Univ Hong Kong, Sch Chinese Med, Fac Med, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Dept Obstet & Gynaecol, Fac Med, Hong Kong, Hong Kong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2016年 / 17卷 / 08期

关键词：

impaired fracture healing; bioinformatics; miRNA; OSTEOCLAST FORMATION; MICRORNA EXPRESSION; OSTEOBLAST DIFFERENTIATION; PROMOTING ANGIOGENESIS; OSTEOPOROTIC FRACTURE; CIRCULATING MIRNAS; BONE REPAIR; INTERLEUKIN-6; OSTEOGENESIS; INHIBITION;

D O I：

10.3390/ijms17081260

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Impaired fracture healing in aged females is still a challenge in clinics. MicroRNAs (miRNAs) play important roles in fracture healing. This study aims to identify the miRNAs that potentially contribute to the impaired fracture healing in aged females. Transverse femoral shaft fractures were created in adult and aged female mice. At post-fracture 0-, 2- and 4-week, the fracture sites were scanned by micro computed tomography to confirm that the fracture healing was impaired in aged female mice and the fracture calluses were collected for miRNA microarray analysis. A total of 53 significantly differentially expressed miRNAs and 5438 miRNA-target gene interactions involved in bone fracture healing were identified. A novel scoring system was designed to analyze the miRNA contribution to impaired fracture healing (RCIFH). Using this method, 11 novel miRNAs were identified to impair fracture healing at 2- or 4-week post-fracture. Thereafter, function analysis of target genes was performed for miRNAs with high RCIFH values. The results showed that high RCIFH miRNAs in aged female mice might impair fracture healing not only by down-regulating angiogenesis-, chondrogenesis-, and osteogenesis-related pathways, but also by up-regulating osteoclastogenesis-related pathway, which implied the essential roles of these high RCIFH miRNAs in impaired fracture healing in aged females, and might promote the discovery of novel therapeutic strategies.

引用

页数：15