Purification and characterization of peach mosaic virus

被引：9
|
作者：
Gispert, C [1 ]
Perring, TM
Creamer, R
机构：
[1] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA
来源：
PLANT DISEASE | 1998年 / 82卷 / 08期
关键词：
D O I：
10.1094/PDIS.1998.82.8.905
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Virus particles were observed by transmission electron microscopy in preparations extracted from symptomatic leaves of Chenopodium amaranticolor that had been mechanically inoculated with peach mosaic virus. The particles were long, flexuous, filamentous rods with an average length of 888 nm. Purified preparations had an A 260/280 nm ratio of 1.25. RNA extracted from purified virus was approximately 8.1 kilobases, and a capsid protein of approximately 27 kDa was found. Polyclonal rabbit antiserum, produced against purified virus, reacted with samples from peach mosaic and cherry mottle leaf-infected plants when used in Western blot analysis.
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页码：905 / 908
页数：4
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