High-Content Screening Technology Combined with a Human Granuloma Model as a New Approach To Evaluate the Activities of Drugs against Mycobacterium tuberculosis

被引：25

作者：

Silva-Miranda, Mayra ^{[1
]}

Ekaza, Euloge ^{[1
]}

Breiman, Adrien ^{[1
]}

Asehnoune, Karim ^{[2
,3
]}

Barros-Aguirre, David ^{[4
]}

Pethe, Kevin ^{[5
]}

Ewann, Fanny ^{[5
]}

Brodin, Priscille ^{[5
,6
]}

Ballell-Pages, Llus ^{[4
]}

Altare, Frederic ^{[1
]}

机构：

[1] Univ Nantes, CNRS, UMR 6299, INSERM U892, Nantes, France

[2] Univ Nantes, Fac Med, Lab UPRES EA3826, Nantes, France

[3] CHU Nantes, Pole Anesthesie Reanimat, Serv Anesthesie Reanimat Chirurg, Hotel Dieu, F-44035 Nantes 01, France

[4] GlaxoSmithKline, Dis Dev World, Madrid, Spain

[5] Inst Pasteur Korea, INSERM Avenir, Songnam, Gyeonggi Do, South Korea

[6] Univ Lille, Inst Pasteur Lille, Ctr Infect & Immun, INSERM U1019,CNRS UMR8204, Lille, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 01期

关键词：

MULTIDRUG-RESISTANT TUBERCULOSIS; PYRAZINAMIDE; MACROPHAGES; INFECTION; DISCOVERY; CELLS;

D O I：

10.1128/AAC.03705-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tuberculosis remains a major health problem due to the emergence of drug-resistant strains of Mycobacterium tuberculosis. Some models have provided valuable information about drug resistance and efficacy; however, the translation of these results into effective human treatments has mostly proven unsuccessful. In this study, we adapted high-content screening (HCS) technology to investigate the activities of antitubercular compounds in the context of an in vitro granuloma model. We observed significant shifts in the MIC(50)s between the activities of the compounds under extracellular and granuloma conditions.

引用

页码：693 / 697

页数：5

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