Polaribacter pacificus sp nov., isolated from a deep-sea polymetallic nodule from the Eastern Pacific Ocean

A Gram-staining-negative, yellow-colony-forming, rod-shaped, non-flagellated and facultatively aerobic strain, designed HRA130-1(T), was isolated from a deep-sea polymetallic nodule from the Pacific Clarion-Clipperton Fracture Zone (CCFZ). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HRA130-1(T) belonged to the genus Polaribacter (96.3-93.2% 16S rRNA gene sequence similarity), and exhibited 94% 16S rRNA gene sequence similarity to Polaribacter filamentus KCTC 23135(T) (type species) and the highest sequence similarity to Polaribacter huanghezhanensis KCTC 32516(T) (96.3 %). Optimal growth occurred in the presence of 4% (w/v) NaCl, at pH 7.0 and 16 degrees C. The DNA G+C content of strain HRA130-1(T) was 35.9 mol%. The major fatty acid was iso-C-15 : 0. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and an unidentified aminolipid. On the basis of data from the present taxonomic study using a polyphasic approach, strain HRA130-1(T) represents a novel species of the genus Polaribacter, for which the name Polaribacter pacificus sp. nov. is proposed. The type strain is HRA130-1(T) (= KCTC 52370(T) = MCCC 1K03199(T) = JCM 31460(T) = CGMCC 1.15763(T)).