Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe

被引：3
|
作者：
Yamauchi, Kosho [1 ]
Matsuoka, Yuta [1 ]
Takahashi, Masatomo [2 ]
Izumi, Yoshihiro [2 ]
Naka, Hideto [1 ]
Taniguchi, Yosuke [3 ]
Kawai, Kazuaki [4 ]
Bamba, Takeshi [2 ]
Yamada, Ken-ichi [1 ]
机构：
[1] Kyushu Univ, Fac Pharmaceut Sci, Phys Chem Life Sci Lab, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan
[2] Kyushu Univ, Res Ctr Trans Med, Med Inst Bioregulat, Metabol Lab,Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan
[3] Kyushu Univ, Fac Pharmaceut Sci, Frontier Biofunct Nucle Acid & Organ Chem, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan
[4] Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Dept Environm Oncol, Yahatanishi Ku, 1-1 Iseigaoka, Kitakyushu, Fukuoka 8078555, Japan
来源：
CHEMICAL COMMUNICATIONS | 2021年 / 58卷 / 01期
关键词：
PARAMAGNETIC-RESONANCE SPECTROSCOPY; TANDEM MASS-SPECTROMETRY; KINETIC APPLICATIONS; RATE CONSTANTS; INDUCED DAMAGE; SPIN; MECHANISMS; OXIDATION;
D O I：
10.1039/d1cc04998d
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.
引用
收藏
页码：56 / 59
页数：5
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