a-Cyperone Ameliorates Lipopolysaccharide-Induced Inflammation and Apoptosis of Periodontal Ligament Stem Cells via Blocking the Toll-Like Receptor 4/NF-?B Signaling

Periodontitis is a long-lasting inflammatory microbial sickness that could cause damage to the periodontal ligament, gums, cementum and alveolar bone directly. Cyperus rotundus is a com-mon traditional Chinese medicine clinically with many pharmacological activities, alpha-Cyperone is a bioactive ingredient abundant in Cyperus rotundus, few studies have focused on its anti-fungal and anti-oxidative stress activities in mice, during this research, we firstly investigated the impact of alpha-Cyperone on PDLSCs inflammatory by LPS stimulation. Our findings illustrated that alpha-Cyperone exerted no influence on PDLSCs viability at concentrations lower than 60 AM, and the release of inflammatory cytokines decreased with an increase in LPS concentration. Meanwhile, we found that alpha-Cyperone effectively restrained LPS-induced PDLSC cell apoptosis by CCK-8 cell viability assay and TUNEL-positive staining. Furthermore, the TLR4 expression and NF-kappa B kinase activa-IP: 182.75.148.10 On: Sat, 20 Aug 2022 10:36:19 tion were greatly repressed by alpha-Cyperone treatment in LPS-indced PDLSCs inflammatory model. Copyright: American Scientific Publishers Subsequently, enhanced TLR4 expressionDeliveredwasyobser vedIngenato reverse the suppressed release of inflammatory cytokines and decreased apoptosis effects mediated by alpha-Cyperone in LPS-cotreated PDLSCs, indicating that the deactivation of TLR4 and downstream NF-KB were implicated in favoring alpha-Cyperone-triggered protective effects of LPS in PDLSCs.