Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica

被引：85
|
作者：
Cao, Xuan [1 ]
Wei, Liu-Jing [1 ]
Lin, Jia-Yu [1 ]
Hua, Qiang [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China
[2] Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2017年 / 245卷
基金：
中国国家自然科学基金;
关键词：
Linalool; Yarrowia lipolytica; Linalool synthase; MVA pathway; SACCHAROMYCES-CEREVISIAE; DIPHOSPHATE SYNTHASE; EXPRESSION; LYCOPENE;
D O I：
10.1016/j.biortech.2017.06.105
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production. A maximum linalool titre of 6.96 +/- 0.29 mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1641 / 1644
页数：4
相关论文
共 50 条
  • [1] Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
    Yinghang Liu
    Xin Jiang
    Zhiyong Cui
    Zhaoxuan Wang
    Qingsheng Qi
    Jin Hou
    Biotechnology for Biofuels, 12
  • [2] Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
    Liu, Yinghang
    Jiang, Xin
    Cui, Zhiyong
    Wang, Zhaoxuan
    Qi, Qingsheng
    Hou, Jin
    BIOTECHNOLOGY FOR BIOFUELS, 2019, 12 (01)
  • [3] Engineering oleaginous yeast Yarrowia lipolytica for production of fuels and chemicals
    Xu, Peng
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 257
  • [4] Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica
    A. Beopoulos
    J. Verbeke
    F. Bordes
    M. Guicherd
    M. Bressy
    A. Marty
    Jean-Marc Nicaud
    Applied Microbiology and Biotechnology, 2014, 98 : 251 - 262
  • [5] Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica
    Beopoulos, A.
    Verbeke, J.
    Bordes, F.
    Guicherd, M.
    Bressy, M.
    Marty, A.
    Nicaud, Jean-Marc
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (01) : 251 - 262
  • [6] Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction
    Shi, Tianqiong
    Li, Yawen
    Zhu, Li
    Tong, Yangyang
    Yang, Junjie
    Fang, Yunming
    Wang, Meng
    Zhang, Jieze
    Jiang, Yu
    Yang, Sheng
    BIOTECHNOLOGY JOURNAL, 2021, 16 (07)
  • [7] Engineering Promoter Architecture in Oleaginous Yeast Yarrowia lipolytica
    Hussain, Murtaza Shabbir
    Gambill, Lauren
    Smith, Spencer
    Blenner, Mark A.
    ACS SYNTHETIC BIOLOGY, 2016, 5 (03): : 213 - 223
  • [8] Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production
    Saez-Saez, Javier
    Wang, Guokun
    Marella, Eko Roy
    Sudarsan, Suresh
    Pastor, Marc Cernuda
    Borodina, Irina
    METABOLIC ENGINEERING, 2020, 62 : 51 - 61
  • [9] Heterologous production of pentane in the oleaginous yeast Yarrowia lipolytica
    Blazeck, John
    Liu, Leqian
    Knight, Rebecca
    Alper, Hal S.
    JOURNAL OF BIOTECHNOLOGY, 2013, 165 (3-4) : 184 - 194
  • [10] Production of abscisic acid in the oleaginous yeast Yarrowia lipolytica
    Arnesen, Jonathan Asmund
    Jacobsen, Irene Hjorth
    Dyekjaer, Jane Dannow
    Rago, Daniela
    Kristensen, Mette
    Klitgaard, Andreas Koedfoed
    Randelovic, Milica
    Martinez, Jose Luis
    Borodina, Irina
    FEMS YEAST RESEARCH, 2022, 22 (01)
12345