RESONANCE LIGHT SCATTERING METHOD FOR MICRODETERMINATION OF PROTEINS BASED ON AGGREGATION OF Au NANOPARTICLES

被引：0
|
作者：
Shu-Hong, Zhang [1 ]
Yong-Shan, Fan [1 ]
Shuo, Feng [2 ]
机构：
[1] Tangshan Teachers Coll, Dept Life Sci, Tangshan 063000, Hebei Province, Peoples R China
[2] Agr Univ Hebei, Coll Sci, Baoding, Hebei, Peoples R China
来源：
ANALYTICAL LETTERS | 2011年 / 44卷 / 04期
关键词：
Au nanoparticles; Proteins; Resonance light scattering; GOLD NANOPARTICLES; NANOGRAM LEVELS; DNA HYBRIDIZATION; ASSAY; IMMUNOASSAY; PRINCIPLE; BINDING; LABELS;
D O I：
10.1080/00032711003783051
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
By using a resonance light scattering (RLS) technique, a highly sensitive method for protein determination based on the aggregation of Au nanoparticles on protein template is described. For the Au nanoparticles of 15 nm, the detection limit of bovine serum albumin was 5.0 ng/mL and the linear range was 10-300 ng/mL. The experimental results indicated that various metal ions do not interfere with this assay. The proposed RLS assay exhibited lower variation in response signals for the same weight of different proteins and showed satisfactory results when it was used for determination of proteins in human serum.
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页码：656 / 666
页数：11
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