Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

被引：0
|
作者：
Noguera, NI
Tallano, CE
Bragós, IM
Milani, AC
机构：
[1] Univ Nacl Rosario, Hosp Prov Centenario, Fac Ciencias Bioquim & Farmaceut, Catedra Hematol, Rosario, Santa Fe, Argentina
[2] Univ Nacl Rosario, Hosp Prov Centenario, Fac Ciencias Bioquim & Farmaceut, Hematol Serv, Rosario, Santa Fe, Argentina
来源：
JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2000年 / 14卷 / 06期
关键词：
DNA extraction; nucleated cells; salting-out; phenol/chloroform; PCR;
D O I：
10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta -globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step. before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples. J. Clin. Lab.Anal. 14:280-283, 2000. (C) 2000 Wiley-Liss, Inc.
引用
收藏
页码：280 / 283
页数：4
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