Antibody-antigen pair probed by combinatorial approach and rational design: Bringing together structural insights, directed evolution, and novel functionality

被引：9

作者：

Belogurov, Alexey, Jr. ^{[1
,2
]}

Smirnov, Ivan ^{[1
,3
]}

Ponomarenko, Natalya ^{[1
]}

Gabibov, Alexander ^{[1
,2
,3
]}

机构：

[1] MM Shemyakin & Yu A Ovchinnikov Inst Bioorgan Che, Moscow, Russia

[2] Inst Gene Biol RAS, Moscow, Russia

[3] Moscow MV Lomonosov State Univ, Dept Chem, Moscow, Russia

来源：

FEBS LETTERS | 2012年 / 586卷 / 18期

基金：

俄罗斯基础研究基金会;

关键词：

Autoantibodies; Autoantigen; Combinatorial screening; Rational design; Reactibodies; Multiple sclerosis; Catalytic vaccine; MYELIN BASIC-PROTEIN; AUTOREACTIVE B-LYMPHOCYTES; IN-VITRO SELECTION; MULTIPLE-SCLEROSIS; CATALYTIC ANTIBODY; CEREBROSPINAL-FLUID; MONOCLONAL-ANTIBODIES; PHAGE DISPLAY; OLIGODENDROCYTE GLYCOPROTEIN; HUMAN BUTYRYLCHOLINESTERASE;

D O I：

10.1016/j.febslet.2012.07.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The unique hypervariability of the immunoglobulin (Ig) superfamily provides a means to create both binding and catalytic antibodies with almost any desired specificity and activity. The diversity of antigens and concept of adaptive response suggest that it is possible to find an antigen pair to any raised Ig. In the current review we discuss combinatorial approaches, which makes it possible to obtain an antibody with predefined properties, followed by 3D structure-based rational design to enhance or dramatically change its characteristics. A similar strategy, but applied to the second partner of the antibody-antigen pair, may result in selection of complementary substrates to the chosen Ig. Finally, 2D screening may be performed solving the "Chicken and Egg" problem when neither antibody nor antigen is known. (C) 2012 Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies.

引用

页码：2966 / 2973

页数：8