Carbonate apatite-facilitated intracellularly delivered siRNA for efficient knockdown of functional genes
被引:69
|
作者:
Hossain, Sharif
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, JapanTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Hossain, Sharif
[1
]
Stanislaus, Anthony
论文数: 0引用数: 0
h-index: 0
机构:
Int Med Univ, Fac Med & Hlth Sci, Kuala Lumpur 57000, MalaysiaTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Stanislaus, Anthony
[2
]
Chua, Ming Jang
论文数: 0引用数: 0
h-index: 0
机构:
Int Med Univ, Fac Med & Hlth Sci, Kuala Lumpur 57000, MalaysiaTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Chua, Ming Jang
[2
]
Tada, Seiichi
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, JapanTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Tada, Seiichi
[1
]
论文数: 引用数:
h-index:
机构:
Tagawa, Yoh-ichi
[1
]
Chowdhury, Ezharul Hogue
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Int Med Univ, Fac Med & Hlth Sci, Kuala Lumpur 57000, MalaysiaTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Chowdhury, Ezharul Hogue
[1
,2
]
Akaike, Toshihiro
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, JapanTokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
Akaike, Toshihiro
[1
]
机构:
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
[2] Int Med Univ, Fac Med & Hlth Sci, Kuala Lumpur 57000, Malaysia
Gene therapy through intracellular delivery of a functional gene or a gene-silencing element is a promising approach to treat critical diseases. Elucidation of the genetic basis of human diseases with complete sequencing of human genome revealed many vital genes as possible targets in gene therapy programs. RNA interference (RNAi), a powerful tool in functional genomics to selectively silence messenger RNA (mRNA) expression, can be harnessed to rapidly develop novel drugs against any disease target. The ability of synthetic small interfering RNA (siRNA) to effectively silence genes in vitro and in vivo, has made them particularly well suited as a drug therapeutic. However, since naked siRNA is unable to passively diffuse through cellular membranes, delivery of siRNA remains the major hurdle to fully exploit the potential of siRNA technology. Here pH-sensitive carbonate apatite has been developed to efficiently deliver siRNA into the mammalian cells by virtue of its high affinity interactions with the siRNA and the desirable size of the resulting siRNA/apatite complex for effective cellular endocytosis. Moreover, following internalization by cells, siRNA was found to be escaped from the endosomes in a time-dependent manner and finally, more efficiently silenced reporter genes at a low dose than commercially available lipofectamine. Knockdown of cyclin B1 gene with only 10 nM of siRNA delivered by carbonate apatite resulted in the significant death of cancer cells, suggesting that the new method of siRNA delivery is highly promising for pre-clinical and clinical cancer therapy. (C) 2010 Elsevier B.V. All rights reserved.
机构:
Purdue Univ, Dept Biomed Engn, W Lafayette, IN 47907 USA
Purdue Univ, Dept Pharmaceut, W Lafayette, IN 47907 USAPurdue Univ, Dept Biomed Engn, W Lafayette, IN 47907 USA
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Tokyo, JapanMonash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
Fukuda, K.
Kutsuzawa, K.
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Tokyo, JapanMonash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
Kutsuzawa, K.
Maruyama, K.
论文数: 0引用数: 0
h-index: 0
机构:
Shizuoka Canc Ctr Res Inst, Shizuoka 4118777, JapanMonash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
Maruyama, K.
Akiyama, Y.
论文数: 0引用数: 0
h-index: 0
机构:
Shizuoka Canc Ctr Res Inst, Shizuoka 4118777, JapanMonash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
Akiyama, Y.
Chowdhury, E. H.
论文数: 0引用数: 0
h-index: 0
机构:
Monash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Tokyo, Japan
Shizuoka Canc Ctr Res Inst, Shizuoka 4118777, JapanMonash Univ, Jeffrey Cheah Sch Med & Hlth Sci, Fac Med Nursing & Hlth Sci, Bandar Sunway, Malaysia
机构:
Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor, MalaysiaMonash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor, Malaysia
Fatemian, Tahereh
Chowdhury, Ezharul Hoque
论文数: 0引用数: 0
h-index: 0
机构:
Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor, MalaysiaMonash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor, Malaysia
机构:
Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, MalaysiaMonash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, Malaysia
Tiash, Snigdha
Kamaruzman, Nur Izyani Binti
论文数: 0引用数: 0
h-index: 0
机构:
Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, MalaysiaMonash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, Malaysia
Kamaruzman, Nur Izyani Binti
Chowdhury, Ezharul Hoque
论文数: 0引用数: 0
h-index: 0
机构:
Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, MalaysiaMonash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway, Malaysia