Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

被引：24

作者：

Menova, Petra ^{[1
]}

Hocek, Michal ^{[1
,2
]}

机构：

[1] Acad Sci Czech Republic, Inst Organ Chem & Biochem, Gilead & IOCB Res Ctr, CZ-16610 Prague 6, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Organ & Nucl Chem, CZ-12843 Prague 2, Czech Republic

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 55期

关键词：

CROSS-COUPLING REACTIONS; NUCLEOSIDE TRIPHOSPHATES; FUNCTIONALIZED DNA; RESTRICTION ENDONUCLEASES; POLYMERASE INCORPORATION; CONSTRUCTION; NUCLEOTIDES; ELECTROCHEMISTRY; CLEAVAGE;

D O I：

10.1039/c2cc32930a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.

引用

页码：6921 / 6923

页数：3

共 13 条

[1] Preparation of modified oligonucleotides by nicking enzyme amplification reaction
Menova, Petra
Hocek, Michal
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 248
[2] Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR
Menova, Petra
Raindlova, Veronika
Hocek, Michal
BIOCONJUGATE CHEMISTRY, 2013, 24 (06) : 1081 - 1093
[3] Fluorescence Quenching in Oligonucleotides Containing 7-Substituted 7-Deazaguanine Bases Prepared by the Nicking Enzyme Amplification Reaction
Menova, Petra
Dziuba, Dmytro
Gueixens-Gallardo, Pedro
Jurkiewicz, Piotr
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Hocek, Michal
BIOCONJUGATE CHEMISTRY, 2015, 26 (02) : 361 - 366
[4] An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplification
Xu, Jingguo
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Li, Xuanxuan
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[5] Preparation of 5′-O-(1-Thiotriphosphate)-Modified Oligonucleotides Using Polymerase-Endonuclease Amplification Reaction (PEAR)
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Dong, Shihua
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PLOS ONE, 2013, 8 (07):
[6] A glucometer-based strategy for sensitive DNA methyltransferase activity detection via a polymerization nicking reaction and enzyme amplification
Chen, Ying
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ANALYTICAL METHODS, 2017, 9 (20) : 2933 - 2938
[7] Terminal deoxynucleotidyl transferase-activated nicking enzyme amplification reaction for specific and sensitive detection of DNA methyltransferase and polynucleotide kinase
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Wang, Si-Yuan
Li, Xiao-Yu
Wang, Ya-Xin
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[8] A NOVEL MEDICAL TECHNOLOGY FOR RAPID MOLECULAR DIAGNOSTICS (NICKING ENZYME AMPLIFICATION REACTION [NEAR]) - A SYSTEMATIC LITERATURE REVIEW, META-ANALYSIS
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VALUE IN HEALTH, 2022, 25 (12) : S384 - S384
[9] Sensitive detection of T4 polynucleotide kinase activity based on coupled exonuclease reaction and nicking enzyme-assisted fluorescence signal amplification
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Analytical and Bioanalytical Chemistry, 2014, 406 : 2943 - 2948
[10] Sensitive detection of T4 polynucleotide kinase activity based on coupled exonuclease reaction and nicking enzyme-assisted fluorescence signal amplification
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Wang, Xiuzhong
Lu, Tingting
Liu, Xiaojuan
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (12) : 2943 - 2948

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