Rhizobium capsici sp nov., isolated from root tumor of a green bell pepper (Capsicum annuum var. grossum) plant

被引:9
|
作者
Lin, Shih-Yao [1 ]
Hung, Mei-Hua [1 ]
Hameed, Asif [1 ]
Liu, You-Cheng [1 ]
Hsu, Yi-Han [1 ]
Wen, Cheng-Zhe [2 ]
Arun, A. B. [3 ]
Busse, Hans-Juergen [4 ]
Glaeser, Stefanie P. [5 ]
Kaempfer, Peter [5 ]
Young, Chiu-Chung [1 ,6 ]
机构
[1] Natl Chung Hsing Univ, Dept Soil & Environm Sci, Coll Agr & Nat Resources, Taichung 402, Taiwan
[2] Natl Chung Hsing Univ, Bachelor Program Bioind Management, Coll Agr & Nat Resources, Taichung 402, Taiwan
[3] Yenepoya Univ, Yenepoya Res Ctr, Mangalore, India
[4] Vet Med Univ, Inst Bakteriol Mykol & Hyg, A-1210 Vienna, Austria
[5] Univ Giessen, Inst Angew Mikrobiol, D-35390 Giessen, Germany
[6] Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung 402, Taiwan
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2015年 / 107卷 / 03期
关键词
Root tumors; Capsicum annuum var. grossum; Legumes; Housekeeping gene; Rhizobium capsici sp nov; CLOSE PHYLOGENETIC RELATIONSHIP; ALLORHIZOBIUM-UNDICOLA; LIPID-COMPOSITION; NIFH GENE; CLASSIFICATION; AGROBACTERIUM; DIVERSITY; BACTERIA; SOIL; DNA;
D O I
10.1007/s10482-014-0371-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel, Gram-staining-negative, rod-shaped, aerobic and motile bacterium, designated strain CC-SKC2(T), was isolated from the root tumor of a green bell pepper (Capsicum annuum var. grossum) plant in Taiwan. Cells were positive for oxidase and catalase activities and exhibited growth at 25-37 A degrees C, pH 4.0-9.0 and tolerated NaCl concentrations up to 4.0 % (w/v). Strain CC-SKC2(T) is able to trigger nodulation in soybean (Glycine max Merr.), but not in Capsicum annuum var. grossum, red bean (Vigna angularis), sesbania (Sesbania roxburghii Merr.) or alfalfa (Medicago varia Martin.). The novel strain shared highest 16S rRNA gene sequence similarity to Rhizobium rhizoryzae KCTC 23652(T) and Rhizobium straminoryzae CC-LY845(T) (both 97.5 %) followed by Rhizobium lemnae L6-16(T) (97.3 %), Rhizobium pseudoryzae KCTC 23294(T) (97.1 %), and Rhizobium paknamense NBRC 109338(T) (97.0 %), whereas other Rhizobium species shared < 96.7 % similarity. The DNA-DNA relatedness values of strain CC-SKC2(T) with R. rhizoryzae KCTC 23652(T), R. pseudoryzae KCTC 23294(T) and R. paknamense NBRC 109338(T) were 11.4, 17.2 and 17.0 %, respectively (reciprocal values were 11.1, 28.3 and 24.0 %, respectively). Phylogenetic analysis based on 16S rRNA, atpD and recA genes revealed a distinct taxonomic position attained by strain CC-SKC2(T) with respect to other Rhizobium species. The major fatty acids in strain CC-SKC2(T) were C-16:0, C-19:0 cyclo omega 8c, C-14:0 3-OH and/or C-16:1 iso I and C-18:1 omega 7c and/or C-18:1 omega 6c. The polyamine pattern showed predominance of spermidine and moderate amounts of sym-homospermidine. The predominant quinone system was ubiquinone (Q-10) and the DNA G+C content was 60.5 mol%. On the basis of polyphasic taxonomic evidence presented here, strain CC-SKC2(T) is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium capsici sp. nov. is proposed. The type strain is CC-SKC2(T) (=BCRC 80699(T) = JCM 19535(T)).
引用
收藏
页码:773 / 784
页数:12
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