Specific detection of RNA on ultra-thin sections

被引：3
|
作者：
Calabuig, J
de Juan, J
Iborra, FJ [1 ]
机构：
[1] John Radcliffe Hosp, Weatherall Inst Mol Med, MRC, Mol Haematol Unit, Oxford OX3 9DS, England
[2] Inst Canc Res, INSTRACAN, Alicante 03400, Spain
[3] Univ Alicante, Fac Ciencias, Dept Biotecnol, E-03080 Alicante, Spain
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 2005年 / 152卷 / 02期
基金：
英国医学研究理事会;
关键词：
electron microscopy; nucleic acid detection; RNA;
D O I：
10.1016/j.jsb.2005.06.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material. (C) 2005 Published by Elsevier Inc.
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页码：146 / 148
页数：3
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