Allele frequencies for 12 autosomal short tandem repeat loci in two Bolivian populations

被引：2

作者：

Cifuentes, L. ^{[1
]}

Jorquera, H. ^{[2
]}

Acuna, M. ^{[1
]}

Ordonez, J. ^{[2
]}

Sierra, A. L. ^{[3
]}

机构：

[1] Univ Chile, Fac Med, Programa Genet Humana, ICBM, Santiago 7, Chile

[2] Genet Technol Ltda, Santiago, Chile

[3] Lab Anal Clin Dr Zuna Ltda, Santa Cruz, Bolivia

来源：

GENETICS AND MOLECULAR RESEARCH | 2008年 / 7卷 / 01期

关键词：

DNA typing; population genetics; short tandem repeat; allele frequencies; forensic science; Bolivian population;

D O I：

10.4238/vol7-1gmr368

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two hundred and sixty unrelated subjects who asked for paternity testing at two Bolivian Laboratories in La Paz and Santa Cruz were studied. The loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, and CSF1PO were typed from blood samples, amplifying DNA by polymerase chain reactions and electrophoresis. Allele frequencies were estimated by simple counting and the unbiased heterozygosity was calculated. Hardy-Weinberg equilibrium was studied and gene frequencies were compared between the two samples. All loci conformed to the Hardy-Weinberg law and allele frequencies were similar in samples from the two cities. The Bolivian gene frequencies estimated were significantly different from those described for Chile and the United States Hispanic-Americans for most of the loci.

引用

页码：271 / 275

页数：5

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