Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells

被引：3
|
作者：
Fomina, S. G. [1 ]
Novikov, D. V. [1 ]
Krasnogorova, N. V. [1 ]
Novikov, V. V. [1 ]
Pleskova, S. N. [1 ]
Karaulov, A. V. [1 ,2 ]
机构：
[1] NI Lobachevsky Nizhny Novgorod Natl Res State Uni, Nizhnii Novgorod, Russia
[2] Sechenov Univ, IM Sechenov Moscow State Med Univ 1, Minist Hlth Russian Federat, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2018年 / 166卷 / 02期
基金：
俄罗斯科学基金会;
关键词：
nanoparticles; quantum dots; reference gene; leukocytes; peripheral blood; POLYMERASE-CHAIN-REACTION; PCR DATA; NORMALIZATION;
D O I：
10.1007/s10517-018-4329-x
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We studied the influence of magnetite nanoparticles (FeO center dot Fe2O3) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.
引用
收藏
页码：264 / 267
页数：4
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