Collective effects in individual oligomers of the red fluorescent coral protein DsRed

被引：39
|
作者：
Cotlet, M
Hofkens, J
Köhn, F
Michiels, J
Dirix, G
Van Guyse, M
Vanderleyden, J
De Schryver, FC
机构：
[1] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium
[2] Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium
来源：
CHEMICAL PHYSICS LETTERS | 2001年 / 336卷 / 5-6期
关键词：
D O I：
10.1016/S0009-2614(01)00165-8
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
We report on single-molecule experiments that were performed on two biological systems, the recently cloned red fluorescent protein of a coral of the Discosoma genus (DsRed) and the enhanced green fluorescent protein, both of which were immobilized in polyvinylalcohol. Fluorescence intensity trajectories were recorded and compared to those of a reference compound, the organic dye hexaphenylbenzene perylenemonoimide. Evidence was found for the presence of DsRed as an oligomer even at subnanomolar concentrations. Both proteins show triplet blinking in the millisecond time range. Collective effects could be observed for the Dsred oligomers. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：415 / 423
页数：9
相关论文
共 50 条
  • [1] The structure of the chromophore within DsRed, a red fluorescent protein from coral
    Gross, LA
    Baird, GS
    Hoffman, RC
    Baldridge, KK
    Tsien, RY
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) : 11990 - 11995
  • [2] Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral
    Baird, GS
    Zacharias, DA
    Tsien, RY
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) : 11984 - 11989
  • [3] Kinetic Analysis of Maturation and Denaturation of DsRed, a Coral-Derived Red Fluorescent Protein
    V. V. Verkhusha
    N. A. Akovbian
    E. N. Efremenko
    S. D. Varfolomeyev
    P. V. Vrzheshch
    Biochemistry (Moscow), 2001, 66 : 1342 - 1351
  • [4] Improved properties of the reef coral red fluorescent protein, drFP583 (DsRed)
    Haugwitz, M
    Terskikh, A
    Fradkov, A
    Dery, O
    Hu, LR
    Nourzaie, O
    Lukyanov, S
    Lukyanov, S
    Angres, B
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 259A - 259A
  • [5] Kinetic analysis of maturation and denaturation of DsRed, a coral-derived red fluorescent protein
    Verkhusha, V.V.
    Akovbyan, N.A.
    Efremenko, E.N.
    Varfolomeev, S.D.
    Vrzheshch, P.V.
    Biokhimiya, 2001, 66 (12): : 1659 - 1670
  • [6] Kinetic analysis of maturation and denaturation of DsRed, a coral-derived red fluorescent protein
    Verkhusha, VV
    Akovbian, NA
    Efremenko, EN
    Varfolomeyev, SD
    Vrzheshch, PV
    BIOCHEMISTRY-MOSCOW, 2001, 66 (12) : 1342 - 1351
  • [7] Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution
    Yarbrough, D
    Wachter, RM
    Kallio, K
    Matz, MV
    Remington, SJ
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) : 462 - 467
  • [8] Photophysics of the Discosoma sp red fluorescent protein DsRed
    Subramaniam, V
    BIOPHYSICAL JOURNAL, 2001, 80 (01) : 7A - 8A
  • [9] Red fluorescent protein (DsRed) as a reporter in Saccharomyces cerevisiae
    Rodrigues, F
    van Hemert, M
    Steensma, HY
    Côrte-Real, M
    Leâo, C
    JOURNAL OF BACTERIOLOGY, 2001, 183 (12) : 3791 - 3794
  • [10] Structural Flexibility of the Monomeric Red Fluorescent Protein DsRed
    Nam, Ki Hyun
    CRYSTALS, 2024, 14 (01)
12345