The Detection and Differentiation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Endocarditis by Using the BD GeneOhm StaphSR Assay

被引：3
|
作者：
Frey, Amy B. [2 ]
Wilson, Deborah A. [2 ]
LaSalvia, Margaret M. [2 ]
Tan, Carmela D. [3 ]
Rodriguez, E. Rene [3 ]
Shrestha, Nabin K. [4 ]
Hall, Gerri S. [2 ]
Procop, Gary W. [1 ]
机构：
[1] Cleveland Clin, Dept Mol Pathol, Cleveland, OH 44195 USA
[2] Cleveland Clin, Dept Clin, Cleveland, OH 44195 USA
[3] Cleveland Clin, Dept Anat, Cleveland, OH 44195 USA
[4] Cleveland Clin, Dept Infect Dis, Cleveland, OH 44195 USA
来源：
AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2011年 / 136卷 / 05期
关键词：
Polymerase chain reaction; PCR; Staphylococcus aureus; Endocarditis; IDENTIFICATION; DIAGNOSIS;
D O I：
10.1309/AJCP9L3BHHGUAQUD
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
We use the BD GeneOhm StaphSR Assay (BD Diagnostics, Oakville, Canada) to screen for Staphylococcus aureus nasal colonization and sought to evaluate this assay for the assessment of valve specimens from patients with endocarditis. We examined 23 paired fresh and formalin-fixed, paraffin-embedded cardiac valve tissue samples, 12 of which had S aureus endocarditis, using the BD GeneOhm StaphSR Assay for the detection and differentiation of methicillin-susceptible and methicillin-resistant S aureus. This assay appropriately characterized all specimens with respect to the presence or absence of S aureus. There was an 87.5% correlation between the presence or absence of the mecA gene and the oxacillin susceptibility results for the S aureus isolates studied. The GeneOhm StaphSR assay accurately detected S aureus in cardiac valve tissue samples. Rare discordances were observed between oxacillin susceptibility status and mecA gene detection by this assay.
引用
收藏
页码：686 / 689
页数：4
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