Synthesis and properties of magnetosensitive nanocomposites and ferrofluids based on magnetite, gemcitabine and HER2 antibody

The processes of adsorption of gemcitabine (GEM) on a surface of nanosized single-domain magnetite (Fe3O4) and magnetic properties of Fe3O4@GEM nanocomposites (NC) have been investigated. Ferrofluids (FF) were synthesized based on magnetite and physiological solution (PS), stabilized with sodium oleate (O1.Na) and polyethylene glycol (PEG), containing GEM (Fe3O4@GEM/O1.Na/PEG+PS). The properties of FF were investigated, as well as cytotoxic/cytostatic activity with respect to HepG2 hepatocellular carcinoma (HCC) cells of human liver. It was revealed that the complex action of GEM and her2 antibody (AB) in composition of FF Fe3O4@GEM/O1.Na/PEG+PS+HER2 onto HepG2 cells caused the synergistic effect and an increase in cytotoxic activity by similar to 18-20 %, compared to GEM in mono-use, with a considerable decrease in its concentration.