Inflammatory myofibroblastic tumour of the urinary bladder: the role of immunoglobulin G4 and the comparison of two immunohistochemical antibodies and fluorescence in-situ hybridization for the detection of anaplastic lymphoma kinase alterations

被引：10

作者：

Choi, Euna ^{[1
]}

Williamson, Sean R. ^{[2
]}

Montironi, Rodolfo ^{[3
]}

Zhang, Shaobo ^{[1
]}

Wang, Mingsheng ^{[1
]}

Eble, John N. ^{[1
]}

Grignon, David J. ^{[1
]}

Lopez-Beltran, Antonio ^{[4
]}

Idrees, Muhammad T. ^{[1
]}

Baldridge, Lee Ann ^{[1
]}

Scarpelli, Marina ^{[3
]}

Jones, Carol L. ^{[1
]}

Wang, Lisha ^{[5
]}

MacLennan, Gregory T. ^{[6
]}

Osunkoya, Adeboye O. ^{[7
]}

Cheng, Liang ^{[1
]}

机构：

[1] Indiana Univ Sch Med, Dept Pathol & Lab Med, 350 West 11th St,IUHPL Room 4010, Indianapolis, IN 46202 USA

[2] Henry Ford Hlth Syst, Dept Pathol, Detroit, MI USA

[3] Polytech Univ Marche Reg Ancona, United Hosp, Sch Med, Inst Pathol Anat & Histopathol, Ancona, Italy

[4] Univ Cordoba, Dept Pathol, Cordoba, Spain

[5] Fudan Univ, Ctr Canc, Dept Pathol, Shanghai 200433, Peoples R China

[6] Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA

[7] Emory Univ, Dept Pathol, Atlanta, GA 30322 USA

来源：

HISTOPATHOLOGY | 2015年 / 67卷 / 01期

关键词：

anaplastic lymphoma kinase (ALK); carcinosarcoma; fluorescence in-situ hybridization (FISH); immunohistochemistry; inflammatory myofibroblastic tumour (inflammatory pseudotumour); molecular genetics; neoplasia; postoperative spindle cell nodule; sarcoma; soft tissue tumour; spindle cell lesions; urinary bladder; CELL LUNG-CANCER; ALK-1 PROTEIN EXPRESSION; NON-HODGKINS-LYMPHOMA; GENITOURINARY TRACT; GENE REARRANGEMENT; CLINICOPATHOLOGICAL FEATURES; EML4-ALK REARRANGEMENT; IGG4-RELATED DISEASE; PLASMA-CELLS; RANBP2; GENE;

D O I：

10.1111/his.12619

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

AimsWe examined gene rearrangement and the expression of anaplastic lymphoma kinase (ALK) in urinary bladder inflammatory myofibroblastic tumour (IMT) using fluorescence in-situ hybridization (FISH) and two immunohistochemical antibodies to ALK. We also investigated whether IMT represents an immunoglobulin (Ig)G4-related disease. Methods and resultsThe performance of the Dako FLEX ALK monoclonal antibody (CD246) and the Cell Signaling Technology ALK (D5F3) XP monoclonal antibody were compared. Overall, 11 of 16 tumours showed ALK expression by immunohistochemistry (69%). Ten demonstrated ALK expression with both stains and one was positive with D5F3 but not CD246 (91% correlation). The D5F3 antibody yielded a stronger staining intensity and a higher sensitivity. Nine tumours demonstrated ALK rearrangements (56%) by FISH. Three were ALK(+) by immunohistochemistry but negative for rearrangement by FISH, whereas one showed rearrangement by FISH but was negative by immunohistochemistry. In total, 12 tumours were positive for ALK abnormalities (75%). Using current criteria, no cases were classified as an IgG4-related disease. ConclusionsThe ALK D5F3 immunohistochemical stain showed superior staining characteristics compared with ALK CD246. Discrepancies in the results between FISH and immunohistochemistry for ALK abnormalities may have causes that are multifactorial. By current criteria, IMT does not represent an IgG4-related disease.

引用

页码：20 / 38

页数：19

共 3 条

[1] Inflammatory Myofibroblastic Tumor of the Urinary Bladder: The Role of IgG4 and the Comparison of Two Immunohistochemical Antibodies and Fluorescence In-Situ Hybridization in the Detection of ALK Alterations
Choi, E.
Williamson, S. R.
Wang, M.
Eble, J. N.
Grignon, D. J.
Montironi, R.
Idrees, M. T.
Zhang, S.
Baldridge, L. A.
Scarpelli, M.
MacLennan, G. T.
Lopez-Beltran, A.
Osunkoya, A. O.
Cheng, L.
LABORATORY INVESTIGATION, 2014, 94 : 221A - 221A
[2] Inflammatory Myofibroblastic Tumor of the Urinary Bladder: The Role of IgG4 and the Comparison of Two Immunohistochemical Antibodies and Fluorescence In-Situ Hybridization in the Detection of ALK Alterations
Choi, E.
Williamson, S. R.
Wang, M.
Eble, J. N.
Grignon, D. J.
Montironi, R.
Idrees, M. T.
Zhang, S.
Baldridge, L. A.
Scarpelli, M.
MacLennan, G. T.
Lopez-Beltran, A.
Osunkoya, A. O.
Cheng, L.
MODERN PATHOLOGY, 2014, 27 : 221A - 221A
[3] Comparison of Reverse Transcription-Polymerase Chain Reaction, Immunohistochemistry, and Fluorescence In Situ Hybridization Methodologies for Detection of Echinoderm Microtubule-Associated Proteinlike 4-Anaplastic Lymphoma Kinase Fusion-Positive Non-Small Cell Lung Carcinoma Implications for Optimal Clinical Testing
Wallander, Michelle L.
Geiersbach, Katherine B.
Tripp, Sheryl R.
Layfield, Lester J.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2012, 136 (07) : 796 - 803

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