Nacre-inspired magnetically oriented micro-cellulose fibres/nano-hydroxyapatite/chitosan layered scaffold enhances pro-osteogenesis and angiogenesis

被引：10

作者：

Ge, Yu-Wei ^{[1
]}

Chu, Min ^{[2
,3
]}

Zhu, Zi-Yang ^{[1
]}

Ke, Qin-Fei ^{[2
,3
]}

Guo, Ya-Ping ^{[2
,3
]}

Zhang, Chang-Qing ^{[1
]}

Jia, Wei -Tao ^{[2
,3
]}

机构：

[1] Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Dept Orthoped Surg, Shanghai 200233, Peoples R China

[2] Shanghai Normal Univ, Key Lab Resource Chem, Educ Minist, Shanghai 200234, Peoples R China

[3] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Shanghai 200234, Peoples R China

来源：

MATERIALS TODAY BIO | 2022年 / 16卷

基金：

中国国家自然科学基金;

关键词：

Nano-hydroxyapatite; Nacre mimicry; Bone regeneration; Angiogenesis; Micro -cellulose fibers; SEGMENTAL BONE DEFECTS; PYRROLOQUINOLINE QUINONE; OXIDATIVE STRESS; TISSUE; COLLAGEN; CHALLENGES; ARCHITECTURE; COMPOSITE;

D O I：

10.1016/j.mtbio.2022.100439

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In situ regeneration of large-segment bone defects is a difficult clinical problem. Here, we innovatively developed magnetically oriented micro-cellulose fibres using nano-hydroxyapatite/chitosan (CEF/Fe3O4/HA/CS) and loaded an NF kappa B pathway inhibitor on the surface of magnetically oriented cellulose fibres (CEF/Fe3O4/HA/CS/PQQ) prepared as a layered bioscaffold. CEF/Fe3O4/HA/CS/PQQ was constructed by layering HA/CS sheets. Nano-hydroxyapatite was deposited on the surface of cellulose fibres, then the magnetic nanoparticles on the cellu-lose fibres were aligned on the surface of chitosan under a magnetic field. Oriented cellulose fibres enhanced the compressive properties of the scaffold, with an average maximum compressive strength of 1.63 MPa. The CEF/ Fe3O4/HA/CS/PQQ layered scaffold was filled into the body, and the acute inflammatory response (IL-1 beta and TNF-alpha) was suppressed through the early sustained release of PQQ. The CEF/Fe3O4/HA/CS/PQQ-layered scaffold further inhibited the osteoclasts differentiation. It was further found that the nano-hydroxyapatite on the surface of oriented cellulose fibres promoted the formation and migration of new blood vessels, accelerated the pro-cessing of collagen-I fibres to cartilage, and endochondral ossification. Hence, the development of the CEF/Fe3O4/HA/CS/PQQ layered scaffold with oriented fibres guides bone growth direction and pro-osteogenesis activity and provides a novel strategy for the in situ regeneration of large segmental bone defects.

引用

页数：15