Stabilizing effect of Azospirillum lectins on β-glucosidase activity

被引:0
|
作者
Alen'kina, S. A. [1 ]
Zharkova, V. R. [1 ]
Nikitina, V. E. [1 ]
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia
关键词
Apply Biochemistry; Chymotrypsin; Azospirillum; Antigenic Property; Azospirillum Brasilense;
D O I
10.1134/S0003683807060026
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lectins from the surface of Azospirillum brasilense Sp7 and Azospirillum brasilense Sp7.2.3 (a mutant with impaired lectin activity) were shown to induce a stabilizing effect on the activity of almond beta-glucosidase under conditions of thermoinactivation and proteolytic enzyme treatment. Differences were revealed in the influence of lectins with various antigenic properties. Our results indicate that the effects of lectins on the catalytic activity of the enzyme are mainly associated with conformational changes in lectin molecules during mutagenesis, but not with carbohydrate specificity (general property). These data should be taken into account in evaluating the role of lectins in the formation of nitrogen-fixing associations.
引用
收藏
页码:583 / 586
页数:4
相关论文
共 50 条
  • [1] Stabilizing effect of Azospirillum lectins on β-glucosidase activity
    S. A. Alen’kina
    V. R. Zharkova
    V. E. Nikitina
    Applied Biochemistry and Microbiology, 2007, 43 : 583 - 586
  • [2] Effect of azospirillum lectins on the activity of proteolytic enzymes and their inhibitors in wheat seedling roots
    Alen'kina, S. A.
    Nikitina, V. E.
    MICROBIOLOGY, 2015, 84 (05) : 630 - 635
  • [3] Effect of azospirillum lectins on the activity of proteolytic enzymes and their inhibitors in wheat seedling roots
    S. A. Alen’kina
    V. E. Nikitina
    Microbiology, 2015, 84 : 630 - 635
  • [4] Effect of lectins from Azospirillum brasilense to peroxidase and oxalate oxidase activity regulation in wheat roots
    Alen'kina, S. A.
    Nikitina, V. E.
    BIOLOGY BULLETIN, 2010, 37 (01) : 89 - 92
  • [5] Effect of lectins from Azospirillum brasilense to peroxidase and oxalate oxidase activity regulation in wheat roots
    S. A. Alen’kina
    V. E. Nikitina
    Biology Bulletin, 2010, 37 : 89 - 92
  • [6] Effect of Azospirillum Lectins on the Activities of Wheat-root Hydrolytic Enzymes
    S. A. Alen’kina
    O. A. Payusova
    V. E. Nikitina
    Plant and Soil, 2006, 283 : 147 - 151
  • [7] Effect of Azospirillum lectins on the activities of wheat-root hydrolytic enzymes
    Alen'kina, S. A.
    Payusova, O. A.
    Nikitina, V. E.
    PLANT AND SOIL, 2006, 283 (1-2) : 147 - 151
  • [8] Effect of Azospirillum Lectins on the Ascorbate Peroxidase Activity and Ascorbic Acid Content in Wheat Seedling Roots Exposed to Abiotic Stresses
    S. A. Alen’kina
    V. E. Nikitina
    Applied Biochemistry and Microbiology, 2020, 56 : 211 - 218
  • [9] Effect of Azospirillum Lectins on the Ascorbate Peroxidase Activity and Ascorbic Acid Content in Wheat Seedling Roots Exposed to Abiotic Stresses
    Alen'kina, S. A.
    Nikitina, V. E.
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2020, 56 (02) : 211 - 218
  • [10] A comparative study of the effect of the lectins of Azospirillum brasilense Sp7 and its mutant on the activity of some enzymes in plant cells
    S. A. Alen’kina
    O. A. Payusova
    V. E. Nikitina
    Microbiology, 2004, 73 : 732 - 733