Y-chromosomal short tandem repeat haplotypes in southern Croatian male population defined by 17 loci

Aim To define the Y-chromosome genetic structure in a sample of men from southern Croatia. Methods Blood samples were collected from 166 unrelated healthy men from southern Croatia at the Department of Forensic Medicine and Biochemical Laboratory of University Hospital Split between 2004 and 2007. Gcnomic DNA was extracted using the standard procedures. Seventeen Y-chromosome short tandem repeat (Y-STR) polymorphic loci (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, GATAH4, DYS437, DYS438, and DYS448) were analyzed using AmpFISTR Yfiler Polymerase Chain Reaction Amplification Kit. Results We observed 152 different haplotypes. Total haplotype diversity was 0.997289 and 141 haplotypes (84.49%) were unique. The most common haplotype was shared by only 4 men in the study sample. The locus diversity ranged between 0.21292 for DYS392 and 0.75546 for DYS439 locus. Conclusion The Y-chromosome structure in men from southern Croatia is very diverse. Combination of Y chromosome 17 STR loci may be used as a powerful tool for individual identification and parentage analysis in the southern Croatian male population.