Oxidative DNA cleavage catalyzed by Mn(III) corroles

Several Mn(III) 10-(hydroxylphenyl)-5, 15-bis(pentafluoro-phenyl) corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2. The catalytic system was composed of 10 mu L 0.5 mg/mL pC DNA 6 in TE buffer solution (pH = 8.0, 4. 0 mol/L Tris, 1. 0 x 10(-3) mol/L EDTA), 10 mu L 1.0x10.(-5) mol/L Mn (III) corrole in DMSO and 10 mu L 0.3% H2O2. After 8 h incubation at room temperature, significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(III) corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn(III) corrole can be used as an artificial model of nuclease.