CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs

被引：12
|
作者：
Dutta, Sisir K. [1 ]
Ghosh, Somiranjan [1 ]
De, Supriyo [1 ]
Hoffman, Eric P. [2 ]
机构：
[1] Howard Univ, Dept Biol, Mol Genet Lab, Washington, DC 20059 USA
[2] Childrens Natl Med Hosp, Ctr Genet Med, Washington, DC 20010 USA
来源：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2008年 / 25卷 / 02期
关键词：
PCB-77; PCB-153; biomarker genes; CYP1A1; MT1K;
D O I：
10.1016/j.etap.2007.10.018
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR. (C) 2007 Published by Elsevier B.V.
引用
收藏
页码：218 / 221
页数：4
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