GTPγS-induced Ca2+ activated Cl- currents:: Its stable induction by Gqα overexpression in Xenopus oocytes

被引：3
|
作者：
Kaibara, M [1 ]
Nagase, Y [1 ]
Murasaki, O [1 ]
Uezono, Y [1 ]
Doi, Y [1 ]
Taniyama, K [1 ]
机构：
[1] Nagasaki Univ, Sch Med, Dept Pharmacol, Nagasaki 8528523, Japan
来源：
JAPANESE JOURNAL OF PHARMACOLOGY | 2001年 / 86卷 / 02期
关键词：
Gq; GTP gamma S; oocyte;
D O I：
10.1254/jjp.86.244
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTP gammaS) (30 mM, 5 nl) did not induce Cl- current in 11 out of 22 oocytes. Injection of increased concentration of GTP gammaS (100 mM, 5 nl) into the oocytes induced Cl- currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gq alpha, GTP gammaS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl- currents. These results indicate that heterogeneous expression of Gq alpha in Xenopus oocytes provides a useful system for studying the functional roles of Gq alpha in regulating cellular events.
引用
收藏
页码：244 / 247
页数：4
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