Effect of Glucose Supplementation in Skim Milk Diluter on Motility, Plasma Membrane and Acrosomal Integrity of Nili-Ravi Buffalo Bull Spermatozoa Stored at 5°C

This experiment was designed to evaluate the effect of glucose supplementation in skim milk diluter on motility, plasma membrane and acrosomal integrity of Nili-Ravi buffalo bull spermatozoa stored at 5 degrees C. Semen from three buffalo bulls was collected and diluted at 37 degrees C with a final concentration of 10 x 10(6) motile spermatozoa per nil in skim milk diluter containing 5, 10 and 30mM glucose or without glucose (control). The diluted semen was cooled to 5 degrees C in 2 hours and stored at 5 degrees C for five days. Motility and plasma membrane integrity of buffalo bull spermatozoa was similar (P > 0.05) in all four experimental diluters at 1(st) day of storage:However, sperm motility and plasma membrane integrity on 3(rd) and 5(th) day of storage was significantly (P < 0.05) higher in diluter containing glucose (5, 10 or 30mM) as compared to control. Sperm acrosomal integrity remained similar (P > 0.05) in all experimental diluters for five days of storage at 5 degrees C. No differences were found among experimental diluters supplemented with 5, I() and 30mM glucose (P<0.05), therefore, 5mM glucose supplementation is recommended. In conclusion, glucose supplementation improved the preservability of Nili-Ravi buffalo bull spermatozoa in skim milk diluter at 5 degrees C.