Effects of 3-methylcholanthrene on the transcriptional activity and mRNA accumulation of the oncogene hWAPL

被引:16
|
作者
Kuroda, M
Oikawa, K
Yoshida, K
Takeuchi, A
Takeuchi, M
Usui, M
Umezawa, A
Mukai, K
机构
[1] Tokyo Med Univ, Dept Pathol, Shinjuku Ku, Tokyo 1608402, Japan
[2] Keio Univ, Shinanomachi Res Pk, Shinjuku Ku, Tokyo 1608582, Japan
[3] Tokyo Med Univ, Dept Ophthalmol, Shinjuku Ku, Tokyo 1600023, Japan
[4] Natl Res Inst Child Hlth & Dev, Setagaya Ku, Tokyo 1548567, Japan
关键词
3-methylcholanthrene (3-MC); hWAPL; uterine cervical cancer; aryl hydrocarbon receptor (AhR); alpha-naphthoflavone (ANF);
D O I
10.1016/j.canlet.2004.08.006
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
hWAPL is a human oncogene associated with uterine cervical cancer. Here, we demonstrate that hWAPL transcription is induced by 3-methylcholanthrene (3-MC) in the cervical carcinoma-derived cell line SiHa. hWAPL transcription was analyzed with evaluation of the mRNA and heterogeneous nuclear RNA (hnRNA) levels by quantitative real time PCR analysis. Flow cytometric analysis suggested that the alteration of hWAPL mRNA levels is independent of cell cycle profile. We also found that DMSO and some components of FBS affect hWAPL transcription. Interestingly, when the aryl hydrocarbon receptor (AhR) function was inhibited by alpha-naphthoflavone (ANF), the induction of hWAPL transcription by 3-MC was greater than that in AhR-functioning normal cells. These observations suggest that there are complex mechanisms regulating the transcription of hWAPL. Furthermore, mRNA level of a mouse homolog of hWAPL in mouse uterus was induced by 3-MC injection into the abdominal cavity. Thus, some effects from 3-MC exposure on uterus may be mediated by the unscheduled overexpression of hWAPL. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:21 / 28
页数:8
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