Reabsorption of chlorophyll fluorescence and its effects on the spectral distribution and the picosecond decay of higher plant leaves

Effects of reabsorption of chlorophyll fluorescence were investigated in measurements on chloroplasts and higher plant leaves. The excitation was performed at 650 nm in a typical excitation/detection arrangement; with increasing chlorophyll concentrations, the measured spectral distribution of the chlorophyll fluorescence was significantly distorted, but no effects on the picosecond decay were observed, when this was detected at 685 nm.