Fingerprinting of cauliflower cultivars using RAPD markers

被引：34
|
作者：
Astarini, IA
Plummer, JA
Lancaster, RA
Yan, GJ
机构：
[1] Univ Western Australia, Fac Nat & Agr Sci, Crawley, WA 6009, Australia
[2] Western Australia Dept Agr, Bunbury Dist Off, Bunbury, WA 6231, Australia
来源：
AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH | 2004年 / 55卷 / 02期
关键词：
Brassica oleracea var. botrytis L; DNA markers; DNA polymorphism; genetic relationships;
D O I：
10.1071/AR03012
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.
引用
收藏
页码：117 / 124
页数：8
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