Vaspin protects mouse mesenchymal stem cells from oxidative stress-induced apoptosis through the MAPK/p38 pathway

被引：11
|
作者：
Zhu, Xiao [1 ]
Zhang, Lingyan [2 ]
Chen, Youming [3 ]
Chen, Bo [4 ]
Huang, Haifeng [5 ]
Lv, Jicheng [1 ]
Hu, Shidi [1 ]
Shen, Jie [1 ]
机构：
[1] Southern Med Univ, Affiliated Hosp 3, Dept Endocrinol & Metab, 183 Tianhe Dist, Guangzhou 510630, Guangdong, Peoples R China
[2] Southern Med Univ, Affiliated Hosp 3, Dept Med Image, Guangzhou 510630, Guangdong, Peoples R China
[3] Southern Med Univ, Affiliated Hosp 3, Dept Med Lab, Guangzhou 510630, Guangdong, Peoples R China
[4] Guangdong Second Prov Hosp, Dept Endocrinol, Guangzhou 510317, Guangdong, Peoples R China
[5] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Internal Med, Guangzhou 510120, Guangdong, Peoples R China
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2019年 / 462卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Vaspin; Mesenchymal stem cells; Apoptosis; ACID-INDUCED APOPTOSIS; KAPPA-B; OSTEOBLASTIC DIFFERENTIATION; OSTEOCLAST FORMATION; CANCER CELLS; HIGH GLUCOSE; BONE; OSTEOPOROSIS; PROLIFERATION; MC3T3-E1;
D O I：
10.1007/s11010-019-03614-8
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The aim of the work was to study the influence of vaspin on oxidative stress-induced apoptosis of mouse mesenchymal stem cells (MSCs). MSCs originated from bone marrow of C57BL/6 mouse were treated with vaspin and/or H2O2 in a dose-dependent manner. Cellular viability detected by CCK-8 and cell apoptosis studied by flow cytometry and TUNEL assay were observed in these cells. The protein expressions of PI3K, p-PI3K, Akt, p-Akt, T-ERK1/2, p-ERK1/2, p38, p-p38, JNK, and p-JNK were tested by Western blot. Vaspin had no significant effect on cellular viability, but significantly reduced H2O2-induced apoptosis. Western blot assay showed that pretreatment with vaspin promoted the activation of p-p38. Inhibition of p38 by SB203580 suppressed the protective effect of vaspin on oxidative stress-induced apoptosis. Vaspin inhibits oxidative stress-induced apoptosis of MSCs via the activation of MAPK/p38 signaling pathway. These findings indicate that vaspin is prone to osteoporosis protection.
引用
收藏
页码：107 / 114
页数：8
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