Identification of essential sequence motifs in the node/notochord enhancer of Foxa2 (Hnf3β) gene that are conserved across vertebrate species

被引：20
|
作者：
Nishizaki, Y [1 ]
Shimazu, K [1 ]
Kondoh, H [1 ]
Sasaki, H [1 ]
机构：
[1] Osaka Univ, Inst Mol & Cellular Biol, Dev Biol Lab, Suita, Osaka 5650871, Japan
来源：
MECHANISMS OF DEVELOPMENT | 2001年 / 102卷 / 1-2期
基金：
日本学术振兴会;
关键词：
mouse; chicken; fish; dwarf gourami; Colisa lalia; conserved non-coding sequences; organizer; node; notochord; floor plate; gut; liver primordium; enhancer; cis-regulatory elements; transgenic mouse; Foxa2; HNF3; beta;
D O I：
10.1016/S0925-4773(01)00281-7
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The expression of a winged-helix transcription factor, Foxa2/HNF3 beta, is essential for development of the node and the notochord. We examined the node/notochord enhancer of mouse Foxa2 for sequence motifs conserved across vertebrate species. We cloned Foxa2 genes from chicken and fish, and identified the respective node/notochord enhancers that were active in transgenic mouse embryos. Comparison of the sequences of the enhancers revealed three evolutionally conserved sequence motifs, CS1, CS2 and CS3. Mutational analysis of the mouse enhancer indicated that CS3 is indispensable for gene expression in the node and the notochord, while CS1 and CS2 are required to augment enhancer activity. These motifs do not correspond to the consensus binding sequences of transcription factors known to be involved in node/notochord development. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：57 / 66
页数：10
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