Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells

被引：78

作者：

Ogawa, T

Tokuda, M

Tomizawa, K

Matsui, H

Itano, T

Konishi, R

Nagahata, S

Hatasa, O

机构：

[1] Kagawa Med Univ, Fac Med, Dept Physiol, Kita Ku, Kagawa 7610793, Japan

[2] Kagawa Med Univ, Fac Med, Dept Maxillofacial Surg, Kita Ku, Kagawa 7610793, Japan

[3] Kagawa Med Univ, Fac Med, Dept Biol, Kita Ku, Kagawa 7610793, Japan

[4] Kagawa Med Univ, Fac Med, Dept Pharmacobioinformat, Kita Ku, Kagawa 7610793, Japan

[5] Okayama Univ, Sch Med, Dept Physiol, Okayama 700, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 249卷 / 01期

关键词：

D O I：

10.1006/bbrc.1998.9118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA. Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 alpha 1 collagen mRNA Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)(2)-vitaminD(3). (C) 1998 Academic Press.

引用

页码：226 / 230

页数：5

共 50 条

[1] THE ACTIVITY OF 22-OXACALCITRIOL IN OSTEOBLAST-LIKE (ROS 17/2.8) CELLS
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